ISSN 0869-6632 (Print)
ISSN 2542-1905 (Online)

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Khlebtsov N. G., Bogatyrev V. A., Dykman L. A., Krasnov Y. M., Melnikov A. G. Optical properties of colloidal-gold bioconjugates. Izvestiya VUZ. Applied Nonlinear Dynamics, 2002, vol. 10, iss. 3, pp. 172-187. DOI: 10.18500/0869-6632-2002-10-3-172-187

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Language: 
English
Heading: 
Innovations in applied physics
Article type: 
Article
UDC: 
535.36
DOI: 
10.18500/0869-6632-2002-10-3-172-187

Optical properties of colloidal-gold bioconjugates

Autors: 
Khlebtsov Nikolai G., Saratov State University
Bogatyrev Vladimir Aleksandrovich, Russian Academy of Sciences' Institute of Biochemistry and Physiology of Plants and Microorganisms
Dykman Lev Abramovich, Russian Academy of Sciences' Institute of Biochemistry and Physiology of Plants and Microorganisms
Krasnov Yaroslav Mikhailovich, Russian Academy of Sciences' Institute of Biochemistry and Physiology of Plants and Microorganisms
Melnikov Andrei Gennadievich, Russian Academy of Sciences' Institute of Biochemistry and Physiology of Plants and Microorganisms
Abstract: 

We discuss optical properties of single ог aggregated colloidal-gold conjugates that саn be fabricated by adsorption of a biopolymer onto the surface of nanoparticles. To simulate extinction аnd scattering of light by such structures, we apply the generalized multisphere Mie solution and the discrete dipole approximation along with а computer model of cluster-cluster aggregation. Our consideration includes the following topics: statistical and orientational averaging of optical observables; optical effects related to the chain-like structures; effects оf polymer coating and interparticle spacing; simulation оf kinetic changes in the optical properties of aggregated sols formed during biospecific binding; modification оf the exact multipole approach for the case of two-layered monomers. In the rest оf this paper, we give а short review of our experimental work оn the topic (including biomedical applications) and provide experimental examples concerning the optical monitoring of biospecific interactions оn а nanometer scale.

Key words: 
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Acknowledgments: 
This work was partially supported by CRDF Grant Ne REC-006, and by RFBR Grants № 01-03-33130 апа № 01-04-48736. We thank D.N. Tychinin (IBPPM RAS) for help in preparation of the manuscript, Yu-lin Xu (Univ. of Florida, USA) and Daniel Mackowski (Auburn Univ., USA) for the GMM computer codes.
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Received: 
21.05.2002
Accepted: 
30.06.2002
Available online: 
12.01.2024
Published: 
30.09.2002
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